Differential surrogate light chain expression governs B-cell differentiation.

Cell Surface Expression Differentiation: Differential Intracellular Versus Surrogate Light Chain Production During B Cell

The omega/lambda 5 surrogate immunoglobulin light chain is expressed on the surface of transitional B lymphocytes in murine bone marrow

The membrane immunoglobulin heavy chain (micron) plays a feedback role during the pre-B stage of B lymphocyte differentiation. In pre-B cell lines, micron associates with two surrogate light chain proteins. The omega chain is disulfide linked to mu and was predicted to be the product of the lambda 5 gene. The iota chain is noncovalently associated with micron. We demonstrate that the omega prot...

The w/X5 Surrogate Immunoglobulin Light Chain Is Expressed on the Surface of Transitional B Lymphocytes in Murine Bone Marrow

The membrane immunoglobulin heavy chain (Am) plays a feedback role during the pre-B stage of B lymphocyte differentiation . In pre-B cell lines, Am associates with two surrogate light chain proteins. The w chain is disulfide linked to p, and was predicted to be the product of the X5 gene. The t chain is noncovalently associated with Am. We demonstrate that the w protein is indeed the product of...

Identification of a Pre-BCR Lacking Surrogate Light Chain

SLP-65-/- pre-B cells show a high proliferation rate in vitro. We have shown previously that lambda5 expression and consequently a conventional pre-B cell receptor (pre-BCR) are essential for this proliferation. Here, we show that pre-B cells express a novel receptor complex that contains a micro heavy chain (microHC) but lacks any surrogate (SL) or conventional light chain (LC). This SL-defici...

Differential effect of an Ig mu transgene on development of pre-B cells in fetal and adult SCID mice.

Progression of pro-B lymphocytes to the pre-B stage depends on the expression of a pre-B cell receptor (pre-BCR), consisting of an Ig mu H chain, Ig surrogate light chain, and associated signal transducing chains. Mice that are unable to express a pre-BCR show an arrest of B cell development at the pro-B stage. Such is the case for severe combined immune deficient (SCID) mice in which mu chains...